Figure 9

Quantification of the SP release was determined by calculating the underneath area of the E protein concentrations from fractions 7 to 10 in Fig. 7 A-G. The underneath areas were normalized by the corresponding total amount of E protein in Fig 7J and the percentage of SP release represented the relative value of each prM mutant (Fig. 7B-G) compared to the wild type (Fig. 7A)