Immunohistochemistry of neuronatin, NF200 (marker for large myelinated A-β fiber neurons), and peripherin (marker for unmyelinated C-fiber and thinly myelinated A-δ fiber neurons) in DRG neurons on post-axotomy day 7. Confocal image of DRG neurons immunostained with anti-neuronatin (red), anti-NF200 (green), and anti-peripherin (green). The double immunoreactivity-positive DRG neurons for neuronatin and peripherin (A) or neuronatin and NF200 (B) in control sham group, and neuronatin and peripherin (C) or neuronatin and NF200 (D) in post-axotomy day 7 group are illustrated. Note that the neurons that were double immunoreactive for Nnat and peripherin were increased in axotomized rats. Scale bar, 50 μm.