Statistical analysis of immunohistochemistry of DRG neurons from control and post-axotomy day 1, 3, 5, and 7 rats. Five to six sections of 10 μm thickness (minimal separation of 90-100 μm) from left L4 or L5 DRG were evaluated. Double immunofluorescence labeling using anti-Nnat, anti-peripherin (marker for unmyelinated C-fiber and thinly myelinated A-δ fiber neurons) and anti-NF200 (marker for large myelinated A-β fiber neurons) antibodies were used to differentiate subpopulation of DRG neurons. Note: The percentage counted for double-labeling was based on the following criteria: in Nnat stained-positive neurons, neurons also stained-positive for peripherin (peripherin(+)) or NF200 (NF200(+)). Value are presented as mean ± S.E.M. * Indicates significant difference between control and post-axotomy rats analyzed by one-way ANOVA with Tukey's post-hoc analysis (P < 0.05, n = 3 for each group).