Figure 4
From: Immobilizing topoisomerase I on a surface plasmon resonance biosensor chip to screen for inhibitors
Surface plasmon resonance sensorgram of the interaction between immobilized topoisomerase I (TopI) and TopI inhibitors. (A) The interaction of camptothecin (CPT) (0~250 nM) with immobilized recombinant hTopI without plasmid DNA in the analytes (upper panel), and with plasmid DNA (1000 ng/mL) in the analytes (lower). (B) The interaction of evodiamine (EVO) (0~125 nM) with immobilized recombinant hTopI without plasmid DNA in the analytes (upper panel), and with plasmid DNA (1000 ng/mL) in the analytes (lower). Data are representative of three independent experiments.