Effects of BFA on AR expression. (A) After cells were cultured with BFA (30 ng/ml), DHT (1 nM), or BFA/DHT combination for 72 h, cell lysates (10 μg) were prepared and analyzed for AR expression using Western blots and autoradiograph of those AR protein bands (110 kDa) is shown. Beta (β)-actin is also shown as a protein loading control. (B) Following the exposure of cells to BFA (30 ng/ml) for 0, 6, 12, or 24 h, the time-dependent reduction in AR expression was analyzed on Western blots. Intensities of AR expressions detected on autoradiograph were quantified using a scan densitometer and expressed by the arbitrary values (*p < 0.05) relative to controls (at 0 h) normalized to 100. The data are mean ± SD from three independent experiments.