Purification of JCV virus-like particles (VLPs). Recombinant JCV VLPs protein in yeast cells was purified and identified by SDS-PAGE (A) and Western blot (B). Lane M: molecular weight marker; Lane VLPs: JCV virus-like particles. The rabbit antibody reactive to the JC virus-like particle is indicated by an arrow. (C) RAW 264.7 cells were pseudo-infected with VLPs packaged with pEGFP-N3 (II, III) and were analyzed by light microscopy (II) and fluorescence microscopy (III). Light microscopy of RAW 264.7 cells with pseudo-infected with VLPs as a control (I).