Effects of VLPs IL-10 shRNA on morphology and viability of RAW 264.7 cells. (A) Two preparations of IL-10 shRNA (IL-10i-1 and IL-10i-2) were packaged into JCV VLPs by osmotic shock. The presence of IL-10 shRNA template in VLPs was confirmed by PCR. Lane 1: VLPs; Lane 2: JCV VLPs with IL-10i-1 shRNA template; Lane 3: VLPs; Lane 4: VLPs with IL-10i-2 shRNA template. (B) RAW 264.7 cells were pseudo-infected with VLPs packaged with IL-10 shRNA and the effects of cell apoptosis were determined by 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI) staining. (C) Cell viability was determined by trypan blue staining. VLPs IL-10 shRNA, VLPs only, or shRNA only did not induce cell death.