In vivo effects of IL-10 shRNA on production of IL-10 and TNF-α in BALB/c mice. Groups of BALB/c mice were intraperitoneally injected with LPS (25 μg). After two hours, mice received VLPs-IL-10 shRNA or irrelevant shRNA intravenously. After 6, 12, 24 and 36 h, serum samples were collected from each mouse and tested for IL-10 and TNF-α levels by ELISA. The level of IL-10 was suppressed after the treatment of IL-10 shRNA. *: P < 0.05; ***: P < 0.005.