Figure 2

Adding FN or LAM during differentiation enhances protein levels of proinsulin and insulin. MSCs were induced by four-stage protocol, and immunohistochemistry was performed for stage IV cells. (A) Immunohistochemistry shows the expression of insulin and proinsulin in stage IV cells. Quantification of IHC staining shows FN and LAM increases the percentage of (B) proinsulin, and (C) insulin positive cells. (mean ± S.D.; ** indicates significant difference (P < 0.01) compared with control by student's t test.) (Bar = 100 μm).