Skip to main content
Figure 5 | Journal of Biomedical Science

Figure 5

From: Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK

Figure 5

Cross-talk between PI3K-Akt and MEK-ERK pathways during differentiation of MSCs into IPCs. (A) MSCs were treated without (DMSO) or with 50 μM of PD98059 or LY294002 and MTT assay were performed at 48 hours. (B) MSCs were pretreated without (DMSO) or with PD98059 or LY294002 at stage III during differentiation with FN or LAM, and western blotting was done for stage IV cells. Quantification of Akt (C) and ERK (D) phosphorylation shows PD98059 and LY294002 increase the activation of Akt and ERK, respectively. Quantitative RT-PCR for (E) insulin and (F) Glut2 expression shows both PD98059 and LY294002 increase the expression of insulin and Glut2. (mean ± S.D.; **indicates significant difference (P < 0.01) compared with control by student's t test.).

Back to article page