Skip to main content
Figure 6 | Journal of Biomedical Science

Figure 6

From: Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK

Figure 6

FN or LAM enhances IPC differentiation by activating Akt and ERK. (A) MSCs were pretreated without (DMSO) or with both PD98059 and LY294002 (PD+LY) at stage III during differentiation with FN or LAM, and western blotting was done for stage IV cells. Quantification of Akt (B) and ERK (C) phosphorylation shows adding both PD98059 and LY294002 decreases the activation of Akt and ERK. (D) Quantitative RT-PCR for insulin and Glut2 expression shows adding both PD98059 and LY294002 decreases the expression of insulin and Glut2. (mean ± S.D.).

Back to article page