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Figure 1 | Journal of Biomedical Science

Figure 1

From: Molecular characterization of the PhoPQ-PmrD-PmrAB mediated pathway regulating polymyxin B resistance in Klebsiella pneumoniae CG43

Figure 1

Deletion effects of ugd , wza and rcsB genes on Klebsiella CPS production and resistance to polymyxin B. (A) Comparison of colony morphology. The K. pneumoniae strains were streaked on an LB agar plate, incubated at 37°C overnight and photographed. (B) Sedimentation test. The strains were cultured overnight in LB broth at 37°C and subjected to centrifugation at 4,000 ×g for 5 min. Quantification of K2 CPS amounts of each strain is shown below the figure. Values are shown as averages ± standard deviations from triplicate samples. (C) Polymyxin resistance assay. The log-phased cultures of K. pneumoniae CG43S3, Δugd, Δwza or ΔrcsB mutants were challenged with 1 or 2 units/ml of polymyxin B. (D) Polymyxin resistance assay. The log-phased culture of K. pneumoniae strains were challenged with 2 or 4 units/ml of polymyxin B. The survival rates are shown as the average ± standard deviations from triplicate samples. *, P < 0.01 compared to the parental strain CG43S3. **, P < 0.01 compared to each strain carrying pRK415.

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