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Figure 2 | Journal of Biomedical Science

Figure 2

From: Molecular characterization of the PhoPQ-PmrD-PmrAB mediated pathway regulating polymyxin B resistance in Klebsiella pneumoniae CG43

Figure 2

Involvement of K. pneumoniae pmrF gene in polymyxin B resistance and intramacrophage survival. (A) The log-phased cultures of K. pneumoniae CG43S3, the ΔpmrF mutant or ΔpmrF carrying pRK415-PmrF were grown in LB or LB supplemented with 1 mM Fe3+ and then challenged with 2 units/ml of polymyxin B. The survival rates are shown as the average ± standard deviations from triplicate samples. (B) The survival rates of K. pneumoniae CG43S3ΔrcsB, the isogenic ΔpmrF ΔrcsB mutant, and ΔpmrF ΔrcsB mutant strain carrying the complementation plasmid pRK415-PmrF within the mouse macrophage RAW264.7 were determined. The results shown are relative survival rates which were calculated from the viable colony counts of intracellular bacteria divided by individual original inoculums. Values are shown as the average of five replicas. Error bars, standard deviations. *, P < 0.01 compared to each parental strain; **, P < 0.01 compared to each mutant strain carrying pRK415-PmrF.

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