Table 2 Oligonucleotide primers used in this study

ppmrF01

5'-GATGGAAAAGCTGAAGGCGATGG-3'

None

-161 relative to the pmrF start codon

ppmrF02

5'-CAGCGATATC ATACCCGGCGTC-3'

Eco RV

+1116 relative to the pmrF start codon

pmrA06

5'-GAGCCATGG TCTATTCCGTG-3'

Nco I

+682 relative to the pmrA start codon

pmrAp03

5'-CAATTGGATCC AGGGCTGTAC-3'

Bam HI

-424 relative to the pmrA start codon

phoP01

5'-CGCTCGCCGTTCGGATCC TG-3'

Bam HI

-171 relative to the phoP start codon

phoP02

5'-GCAACGGTACC TTCATCAGCGC-3'

Kpn I

+729 relative to the phoP start codon

pmrDe02

5'-CGAGCTC GTGTTATTTGTCGGCGTTTGTC-3'

Sac I

+250 relative to the pmrD start codon

pmrDp01

5'-TGGATCC TTCATGACGCTCTCTC-3'

Bam HI

-278 relative to the pmrD start codon

pmrDp02

5'-CGCACAGATCT GAAGCACGAC-3'

Bgl II

+75 relative to the pmrD start codon

pmrHp01

5'-TCTGGATCC TGGTCATTAATTGCCCGGC-3'

Bam HI

-425 relative to the pmrH start codon

pmrHp02

5'-CTTAGATCT CGCTCATCATCATCCTGTTC-3'

Bgl II

+34 relative to the pmrH start codon

phoP05

5'-GTAATGACAGCGGGAAGATATG-3'

None

+753 relative to the phoP start codon

phoP06

5'-CAGCCGTTTATATTTTGCGT-3'

None

-25 relative to the phoP start codon

pmrBe03

5'-TGGATCC TCGCAAGATCACCCGCC-3'

Bam HI

+283 relative to the pmrB start codon

pmrBe04

5'-CAAGCTT ATGGGTGCTGACGTTCTGAC-3'

Hin dIII

+1095 relative to the pmrB start codon

KP1760-1

5'-GGAATTCCATATG AAAATCTTAGTCATTGAA-3'

Nde I

+1 relative to the pmrA start codon

KP1760-2

5'-CCGCTCGAG CTATTCCGTGTCGATGTTGTT-3'

Xho I

+672 relative to the pmrA start codon

KP3573-1

5'-GGAATTCCATATG GAGTGGTGGGTAAAAAAA-3'

Nde I

+1 relative to the pmrD start codon

KP3573-2

5'-CCGCTCGAG TTTGTCGGCGTTTGTCCAACG-3'

Xho I

+243 relative to the pmrD start codon

pmrA10

5'-ACTCGAG CCATGGTCTATTCCGTG-3'

Xho I

+1 relative to the pmrA start codon

pmrA11

5'-AATGCGGCCGC AATGAAAATCTTAGTC-3'

Not I

+672 relative to the pmrA start codon

pmrDe15

5'-AAAGCGGCCGC GATGGAGTGGTGGGTAAAAAAAGTA-3'

Not I

+1 relative to the pmrD start codon

pmrDe16

5'-TTTCTCGAG TGTGTTATTTGCCGGCGTTT-3'

Xho I

+243 relative to the pmrD start codon