Figure 2
Flow cytometry analysis of MOSEC-GFP tumor cells incubated with MOSEC-dox cells mixed together or separated by a transwell membrane. MOSEC cells incubated for 2 hrs in with doxorubicin (MOSEC-dox) at a concentration of 100 μg/ml were added to MOSEC-GFP cells (5 × 105/well) in various amounts according to the indicated ratios. Another pool of MOSEC-dox cells (5 × 104) were added to the upper plate of a transwell system with 5 × 105 MOSEC-GFP cells in the bottom plate. A transwell system in which the MOSEC-dox cells were mixed together with MOSEC-GFP cells was used as a control. At 24 hrs of culture, the cells from the mixtures and from the bottom plate of the transwell system were collected and analyzed for presence of GFP and doxorubicin using flow cytometry. Representative figures from the flow cytometry data of (A) the different mixtures of MOSEC-GFP cells incubated with differing amounts MOSEC-dox cells and (B) bottom well containing MOSEC-GFP cells of the transwell system, with data from the control mixture to the right. The numbers in the upper right hand corner show the percentage of total collected cells that indicate presence of GFP and doxorubicin.