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Figure 3 | Journal of Biomedical Science

Figure 3

From: Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines

Figure 3

Versatile differentiation potential of 3A6. (A) Morphology without induction or with osteogenic (21 days, demonstrated by von Kossa staining), chondrogenic (21 days, demonstrated by Alcian Blue staining) or adipogenic (14 days, demonstrated by Oil Red O staining) differentiation. (B) Neural differentiation confirmed by the alteration of cell morphology to the round cell body with bipolar elongated cell processes, and by RT-PCR after induction with noggin for 14 days. (C) Cobble stone formation by co-culture with hematopoietic stem cells. (D) Trophoectoderm- and (E) germline-differentiation analyzed by RT-PCR after induction in three individual clones with BMP4 and RA, respectively.

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Journal of Biomedical Science

ISSN: 1423-0127