Figure 3
From: Enterovirus type 71 2A protease functions as a transcriptional activator in yeast
Western blotting analysis of wild-type EV71 2A protease or with amino acid 110 mutation from Cys to Ala in HeLa cells. HeLa cells were transfected with vector only (lane 1), or with the plasmid encoding the C-terminus of VP1 and wild-type 2A (lane 2), or with the plasmid encoding the C-terminus of VP1 and 2A with amino acid 110 mutation from Cys to Ala (lane 3). After transfection, cell lysates were analyzed and detected using rabbit anti-EV71 2A protein polyclonal antibody (A), mouse anti-eIF4G monoclonal antibody (B), or mouse anti-PARP monoclonal antibody (C). The thin arrows indicate the uncleaved proteins (VP1-2A, intact eIF4G, or intact PARP) while the thick arrows indicate the cleaved products (2A, cleaved eIF4G, or cleaved PARP).