Skip to main content

Table 1 PCR primers used in this study

From: Enterovirus type 71 2A protease functions as a transcriptional activator in yeast

Name Sequence
2AY-S (5'-GGAATTC GGGAAATTTGGACAG-3')
2AY-AS (5'-CCGCTCGAG TTA CTGCTCCATGGCTTC-3')
2AY-21S (5'-GGAATTC CATCTTGCTACTCATAA-3')
2AY-41S (5'-GGAATTC CTCGTATCATCTACCAC-3')
2AY-61S (5'-GGAATTC GGAGTGTATTATTGTAA-3')
2AY-90AS (5'-TTATTA ATAATACTCGCTGGCCTC-3')
2AY-110AS (5'-TTATTA GCAATCCCCTGGTTCCGA-3')
2AY-130AS (5'-TTATTA GCAATCCCCTGGTTCCGA-3')
VP1/2A-S (5'-CCATCGAT ATG ATGGGTACGTTC-3')
2A-S10 (5'-GGAATTC ATG GGGAAATTTGGACAGCAG-3')
2A-AS2 (5'-GCTCTAGA CTA CTGCTCCATGGCTTCATCATC-3')
2A-AS3 (5'-GCTCTAGA CTGCTCCATGGCTTCATCATC-3')
C110A-S (5'-CCAGGGGATGCC GGTGGCATTCTTAGATGC-3')
C110A-AS (5'-AATGCCACCGGC ATCCCCTGGTTCCGAATG-3')
L30/43-S (5'-CATAATGACTGGGCAAACTCATCTACCACTGCTCAA-3')
L30/43-AS (5'-TTGAGCAGTGGTAGATGAGTTTGCCCAGTCATTATG-3')
2AY-AS101 (5'-CCGCTCGAGTTA CTGATCATCCAACCACAGAAG-3')
2A-AS301 (5'-GCTCTAGA CTGATCATCCAACCACAGAAG-3')
CoxB2AY-S (5'-GGAATTC ATG GGACAACAATCAGGGGC-3')
CoxB2AY-AS (5'-TTATTA CTGTTCCATTGCATCATC-3')
CoxB2AY-61S (5'-GGAATTC TTTTGTGCGTCCAAAAAC-3')
CoxB2AY-127AS (5'-TTATTA GCCTTCACCCCCCATGGT-3')
PCBP2-S (5'-CTCTCACCATCCGGCTACTTAT-3')
PCBP2-AS (5'-GCTGCTTATGTCCTCTTCCAGT-3')
PTBP1-S (5'-CTACATCCAGTTCTCCAACCAC-3')
PTBP1-AS (5'-GCTGCTTATGTCCTCTTCCAGT-3')
RTN3-S (5'-ACTCTGTCCTCAGAAGCTTTCC-3')
RTN3-AS (5'-CTCATAGACAATCGGGACACTG-3')
GBF1-S (5'-CCCACTATTGCTGCTCTCTCTT-3')
GBF1-AS (5'-CTGGGCAGGTTCTCAATAGACT-3')
CD55-S (5'-CCGTCTTCTATCTGGTTCTCGT-3')
CD55-AS (5'-GTTACTAGCGTCCCAAGCAAAC-3')
SAM68-S (5'-CGAAGGCTATTACAGCCAGAGT-3')
SAM68-AS (5'-CATATGGGTGCTCTCTGTATGC-3')
  1. Note: Nucleotides for restriction enzyme cutting sites are italicized. Nucleotides for point mutations are bold and italicized. Nucleotides for start and stop codons are marked with bold letters. Primers for the detection of cellular genes were used in real-time RT-PCR.