Figure 2
From: Hypoglycemia induced changes in cholinergic receptor expression in the cerebellum of diabetic rats
Representative graph showing Real Time PCR amplification of muscarinic M3 mRNA from the cerebellum of Control, Diabetic, Diabetic + IIH and Control + IIH Rats. The ΔΔCT method of relative quantification was used to determine the fold change in expression with β-actin CT value as the internal control and Control CT value as the calibrator. C- Control, D- Diabetic, D + IIH - Insulin induced hypoglycemia in diabetic, C + IIH - Insulin induced hypoglycemia in control. Values are mean ± S.D. of 4-6 separate experiments. Each group consisted of 6-8 rats. a p < 0.001 when compared to control. b p < 0.001 when compared to Diabetic.