Representative graph showing Real Time PCR amplification of ChAT mRNA from the cerebellum of Control, Diabetic, Diabetic + IIH and Control + IIH Rats. The ΔΔCT method of relative quantification was used to determine the fold change in expression with β-actin CT value as the internal control and Control CT value as the calibrator. Values are mean ± S.D. of 4-6 separate experiments. Each group consisted of 6-8 rats. a p < 0.001 when compared to control, b p < 0.001 when compared to Diabetic.