Figure 5

Transcriptional activation of HO-1 leads to preferential upregulation of NOS I/PKG signaling in RVLM. Illustrative gels or summary of fold changes against aCSF controls in ratio of nitric oxide synthase I (NOS I), protein kinase G (PKG), NOS II or nitrotyrosine (NT) relative to β-actin protein detected in ventrolateral medulla of rats that received immunoneutralization of HO-1 or HO-2, or knockdown of ho-1 or ho-2 gene in RVLM, 1 h or 24 h before induction of Mev intoxication. Note that NT is presented as % relative to β-actin because it is below detection limit (ND) in aCSF controls. Values are mean ± SEM of triplicate analyses on samples pooled from 4-6 animals per experimental group. *P < 0.05 versus aCSF group and ⁺P < 0.05 versus Mev group in the Scheffé multiple-range test.