Figure 1
From: Membrane proteomic analysis of pancreatic cancer cells
Analysis and identification of membrane proteins in AsPC-1 and BxPC-3 cells using a proteomics approach based on SDS-PAGE, in-gel digestion and LC-MS/MS. (A) Membrane proteins were isolated, separated with SDS-PAGE and detected with Simply Blue stain. The gel bands were then excised and digested with trypsin, and the resulting peptides were extracted for LC-MS/MS analysis. (B) 221 and 208 proteins were identified from AsPC-1 and BxPC-3 cells, respectively, with 109 proteins present in both cell lines.