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Table 2 Metabolic enzymes identified in AsPC-1 cells

From: Membrane proteomic analysis of pancreatic cancer cells

Protein name

Accession #

Unique peptides

Total peptides

Mr (Kda)

PI

Biological process

2-oxoglutarate dehydrogenase E1 component, mitochondrial precursor

ODO1_HUMAN

8

18

115.9

6.39

Glycolysis

3,2-trans-enoyl-CoA isomerase, mitochondrial precursor

D3D2_HUMAN

3

13

32.8

8.8

Fatty acid metabolism; Lipid metabolism

3-hydroxyacyl-CoA dehydrogenase type-2

HCD2_HUMAN

6

10

26.9

7.65

Lipid metabolic process; tRNA processing

3-hydroxyisobutyrate dehydrogenase, mitochondrial precursor

3HIDH_HUMAN

7

16

35.3

8.38

Pentose-phosphate shunt; valine metabolic process

3-ketoacyl-CoA thiolase, peroxisomal precursor

THIK_HUMAN

3

4

44.3

8.76

Fatty acid metabolism; Lipid metabolism

3-mercaptopyruvate sulfurtransferase

THTM_HUMAN

3

7

33.2

6.13

Cyanate catabolic process

78 kDa glucose-regulated protein

GRP78_HUMAN

7

12

72.3

5.07

ER-associated protein catabolic process; ER unfolded protein response; ER regulation of protein folding

Acetyl-CoA acetyltransferase, mitochondrial precursor

THIL_HUMAN

2

6

45.2

8.98

Ketone body metabolism

Aconitate hydratase, mitochondrial

ACON_HUMAN

2

3

85.4

7.36

Tricarboxylic acid cycle

Acyl-protein thioesterase 1

LYPA1_HUMAN

2

2

24.7

6.29

Fatty acid metabolism; Lipid metabolism

Adenylate kinase 2, mitochondrial

KAD2_HUMAN

7

20

26.5

7.67

Nucleic acid metabolic process

ADP/ATP translocase 2

ADT2_HUMAN

5

11

32.9

9.76

Transmembrane transporter activity

Aldehyde dehydrogenase, mitochondrial

ALDH2_HUMAN

3

7

56.3

6.63

Alcohol metabolic process

Alpha-enolase

ENOA_HUMAN

2

2

47.1

7.01

Glycolysis

Amine oxidase B

AOFB_HUMAN

2

2

58.7

7.2

Oxidation reduction

Aspartate aminotransferase, mitochondrial

AATM_HUMAN

4

6

47.4

9.14

Lipid transport

ATP synthase subunit alpha, mitochondrial

ATPA_HUMAN

21

52

59.7

9.16

ATP synthesis

ATP synthase subunit d, mitochondrial

ATP5H_HUMAN

3

7

18.5

5.21

ATP synthesis; Ion transport

ATP synthase subunit b, mitochondrial

AT5F1_HUMAN

2

3

28.9

9.37

ATP synthesis

ATP synthase subunit beta, mitochondrial

ATPB_HUMAN

28

95

56.5

5.26

ATP synthesis

ATP synthase subunit f, mitochondrial

ATPK_HUMAN

2

2

10.9

9.7

ATP synthesis; Ion transport

ATP synthase subunit gamma, mitochondrial;

ATPG_HUMAN

3

6

33

9.23

ATP synthesis; proton transport

ATP synthase subunit O, mitochondrial

ATPO_HUMAN

6

11

23.3

9.97

ATP synthesis, ion transport; ATP catabolic process

Calcium-binding mitochondrial carrier protein Aralar2

CMC2_HUMAN

7

16

74.1

7.14

Mitochondrial aspartate and glutamate carrier

Citrate synthase, mitochondrial precursor

CISY_HUMAN

2

3

51.7

8.45

Tricarboxylic acid cycle

Cytochrome b5 type B

CYB5B_HUMAN

2

4

16.3

4.88

Electron transport

Cytochrome b-c1 complex subunit 1, mitochondrial

QCR1_HUMAN

6

12

52.6

5.94

Electron transport

Cytochrome b-c1 complex subunit 2, mitochondrial

QCR2_HUMAN

3

4

48.4

8.74

Aerobic respiration; electron transport chain; oxidative phosphorylation

Cytochrome c oxidase subunit 2

COX2_HUMAN

2

6

25.5

4.67

Electron transport chain

Cytochrome c1, heme protein, mitochondrial

CY1_HUMAN

5

10

35.4

9.15

Electron transport chain

Cytochrome c1, heme protein, mitochondrial

CY1_HUMAN

2

3

35.4

9.15

Electron transport chain

D-beta-hydroxybutyrate dehydrogenase, mitochondrial precursor

BDH_HUMAN

2

3

38.1

9.1

Oxidation reduction

Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial

ECH1_HUMAN

4

10

35.8

8.16

Fatty acid metabolism; Lipid metabolism

Delta-1-pyrroline-5-carboxylate synthetase

P5CS_HUMAN

2

4

87.2

6.66

Amino-acid biosynthesis; Proline biosynthesis

Dihydrolipoyl dehydrogenase, mitochondrial

DLDH_HUMAN

7

16

54.1

7.95

Cell redox homeostasis

Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

ODP2_HUMAN

3

5

65.7

7.96

Glycolysis

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial

ODO2_HUMAN

4

7

48.6

9.01

Tricarboxylic acid cycle

Electron transfer flavoprotein subunit alpha, mitochondrial

ETFA_HUMAN

2

5

35.1

8.62

Electron transport

Electron transfer flavoprotein subunit beta

ETFB_HUMAN

4

6

27.8

8.25

Electron transport

Endoplasmin

ENPL_HUMAN

16

28

92.4

4.76

ER-associated protein catabolic process; protein folding/transport; response to hypoxia

Enoyl-CoA hydratase, mitochondrial

ECHM_HUMAN

9

26

31.4

8.34

Fatty acid metabolism; Lipid metabolism

Glutamate dehydrogenase 1, mitochondrial;

DHE3_HUMAN

3

4

61.4

7.66

Glutamate metabolism

Glyceraldehyde-3-phosphate dehydrogenase

G3P_HUMAN

5

7

36

8.57

Glycolysis

Glycerol-3-phosphate dehydrogenase, mitochondrial precursor

GPDM_HUMAN

8

15

80.8

7.23

Glycolysis

Haloacid dehalogenase-like hydrolase domain-containing protein 3

HDHD3_HUMAN

3

4

28

6.21

Metabolic process phosphoglycolate phosphatase activity

Histidine triad nucleotide-binding protein 2

HINT2_HUMAN

2

3

17.2

9.2

Lipid synthesis; Steroid biosynthesis

Hyaluronidase-3

HYAL3_HUMAN

2

2

46.5

 

Carbohydrate metabolic process

Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial precursor

HCDH_HUMAN

2

4

34.3

8.88

Fatty acid metabolism; Lipid metabolism

Isoleucyl-tRNA synthetase, mitochondrial precursor

SYIM_HUMAN

5

7

113.7

6.78

Protein biosynthesis

Isovaleryl-CoA dehydrogenase, mitochondrial

IVD_HUMAN|

2

2

46.3

8.45

Leucine catabolic process; Oxidation reduction

L-lactate dehydrogenase A chain

LDHA_HUMAN

3

5

36.7

8.84

Glycolysis

Lon protease homolog, mitochondrial

LONM_HUMAN

2

2

106.4

6.01

Required for intramitochondrial proteolysis

Long-chain-fatty-acid--CoA ligase 5;

ACSL5_HUMAN

2

4

75.9

6.49

Fatty acid metabolism; Lipid metabolism

Malate dehydrogenase, mitochondrial

MDHM_HUMAN

3

5

35.5

8.92

Tricarboxylic acid cycle; Glycolysis

Medium-chain specific acyl-CoA dehydrogenase, mitochondrial

ACADM_HUMAN

2

6

46.6

8.61

Fatty acid metabolism; Lipid metabolism

Mitochondrial carrier homolog 2

MTCH2_HUMAN

3

10

33.3

8.25

Transmembrane transport

Mitochondrial inner membrane protein

IMMT_HUMAN

2

2

83.6

6.08

Protein binding; Cell proliferation-inducing

NADH-cytochrome b5 reductase 3

NB5R3_HUMAN

3

3

34.2

7.18

Cholesterol biosynthesis; Lipid/steroid synthesis

Neutral alpha-glucosidase AB

GANAB_HUMAN

6

9

106.8

5.74

Carbohydrate metabolic process

Peptidyl-prolyl cis-trans isomerase A

PPIA_HUMAN

2

3

18

7.68

Protein folidng; Interspecies interation

Peroxiredoxin-5

PRDX5_HUMAN

2

5

22

8.85

Cell redox homeostasis

Phosphoenolpyruvate carboxykinase, mitochondrial

PPCKM_HUMAN

8

18

70.6

7.56

Gluconeogenesis

Phosphoglycerate kinase 1

PGK1_HUMAN

4

7

44.6

8.3

Glycolysis

Protein disulfide-isomerase

PDIA1_HUMAN

3

3

57.1

4.76

Cell redox homeostasis

Protein disulfide-isomerase A3

PDIA3_HUMAN

4

7

56.7

5.98

Cell redox homeostasis

Protein disulfide-isomerase A4

PDIA4_HUMAN

2

2

72.9

4.96

Cell redox homeostasis; Protein secretion

Protein disulfide-isomerase A6

PDIA6_HUMAN

2

3

48.1

4.95

Cell redox homeostasis; Protein folding

Protein ETHE1, mitochondrial

ETHE1_HUMAN

4

11

27.9

6.35

Metabolic homeostasis in mitochondria

Protein transport protein Sec16A

SC16A_HUMAN

2

2

233.4

5.4

ER-Golgi transport; Protein transport

Pyruvate dehydrogenase E1 component alpha subunit, somatic form

ODPA_HUMAN

2

4

43.3

8.35

Glycolysis

Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial precursor

ODPAT_HUMAN

3

7

42.9

8.76

Glycolysis

Pyruvate dehydrogenase E1 component subunit beta, mitochondrial

ODPB_HUMAN

2

3

39.2

6.2

Glycolysis; Tricarboxylic acid cycle

Serine hydroxymethyltransferase, mitochondrial

GLYM_HUMAN

12

21

56

8.76

L-serine metabolic process; Glycine metabolic process; One-carbon metabolic process

Succinate dehydrogenase flavoprotein subunit, mitochondrial

DHSA_HUMAN

2

5

72.6

7.06

Electron transport; Tricarboxylic acid cycle

Succinyl-CoA ligase [GDP-forming] beta-chain, mitochondrial precursor

SUCB2_HUMAN

3

3

46.5

6.15

Succinyl-CoA metabolic process; Tricarboxylic acid cycle

Succinyl-CoA ligase [GDP-forming] subunit alpha, mitochondrial precursor

SUCA_HUMAN

2

5

35

9.01

Tricarboxylic acid cycle

Superoxide dismutase [Mn], mitochondrial

SODM_HUMAN

2

5

24.7

8.35

Elimination of radicals

Thioredoxin-dependent peroxide reductase

PRDX3_HUMAN

4

10

27.7

7.68

Cell redox homeostasis; Hydrogen peroxide catabolic process

Thiosulfate sulfurtransferase

THTR_HUMAN

2

3

33.4

6.77

Cyanate catabolic process

Trifunctional enzyme subunit alpha, mitochondrial

ECHA_HUMAN

17

46

82.9

9.16

Fatty acid metabolism; Lipid metabolism

Trifunctional enzyme subunit beta, mitochondrial

ECHB_HUMAN

6

12

51.3

9.45

Fatty acid metabolism

Trimethyllysine dioxygenase, mitochondrial

TMLH_HUMAN

2

3

49.5

7.64

Carnitine biosynthesis

Very long-chain specific acyl-CoA dehydrogenase, mitochondrial

ACADV_HUMAN

3

5

70.3

8.92

Fatty acid metabolism; Lipid metabolism

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Journal of Biomedical Science

ISSN: 1423-0127