Figure 10
Confocal image of 5-HTT receptors in the hippocampus of control and Experimental rats using immunofluorescent 5-HTT receptor specific primary antibody and FITC as secondary antibody. The pixel intensity of control - 4235653 ± 1960, diabetic - 2833408 ± 1978 a, diabetic+Insulin - 3964668 ± 1670a, b, diabetic+pyridoxine - 2897587 ± 3426a, diabetic+insulin+pyridoxine - 4121017 ± 2723b, diabetic+Aegle marmelose - 3866844 ± 1534a, b and diabetic+Aegle marmelose+ pyridoxine - 4098789 ± 2086 b. Values are mean ± S.E.M of 4-6 rats in each group. Each group consists of 6-8 rats. a p < 0.001 when compared to control group; b p < 0.001 when compared to diabetic group. There was significant reversal of 5-HTT receptor to near control level on treatment with pyridoxine alone and in combination therapy with insulin and Aegle marmelose compared to diabetic rats. Arrow in white shows 5-HTT.