Three novel beta-galactosidase gene mutations in Han Chinese patients with GM1 gangliosidosis are correlated with disease severity

Table 2 Primers used for site-directed mutagenesis

Genetic variation	Amino acid change	Exon	Oligonucleotides (5' -> 3')
c.304C > G	p.His102Asp	3	F: AACTGGTACTGTCCTGGCCAGGGCTCATGAAAGTTCCAG
			R: CCTGGCCAGGACAGTACCAGTTTTCTGAGGACG ATGATGTGGAATATTTTC
c.495_497delTCT	p. L166del	5	F: ACCACTTGTCCACAGCTGCCAGGTAATCTGGGTCGGAGG
			R: CTGGCAGCTGTGGACAAGTGGTTGGGAGTCCT---GCCCAAGATGAAGCCTC
c.902C > T	p.Ala301Val	8	F: AAGTATATCATAGAGGGAGGAAGCCACT GCTTCGGTCTTG
			R: TTCCTCCCTCTATGATATACTTGCCCGTGGGGTGAGTGTGAACTTGTACATG
c.1481G > T	p.Gly494Val	15	F: GTTGATATATGCACCATAGTTCACACGTCCCATGTTCTC
			R: GAACTATGGTGCATATATCAACGATTTTAAGGT TTTGGTTTCTAACCTG

¹Nucleotide changes are marked with an underline (nucleotide substitution) or a string of hyphens (deletion).

ISSN: 1423-0127