Figure 5

Cre-mediated recombination in the ventral diencephalon in the e11.5 embryo. Epifluorescence images of the e11.5 diencephalon of pdx1-cre^Cre/0; Zeg^GFP/0 embryos, transversely sectioned, immunostained for GFP (green) and Isl1 (A) or Mash1 (B) (red). GFP was not expressed at the ventral surface near the floor plate adjacent to the Isl1⁺ Mash1⁺ neurogenic zone. Scale bars, 100 μm.