Figure 7

Lack of detectable expression of endogenous pdx1 in the mouse hindbrain. Section from the r1 region of a pdx1-cre^Cre/0; Zeg^GFP/0 embryo, immunostained for GFP (A) and Pdx1 (B) using TSA amplification. No detectable expression of Pdx1 was observed, despite robust expression of GFP. Scale bars, 100 μm.