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Table 1 Transgenic mouse lines and relevant genotypes employed

From: Precise pattern of recombination in serotonergic and hypothalamic neurons in a Pdx1-cre transgenic mouse line

Formal designation Common designation Transgene design Trans-gene insertion Purpose WT allele Trans-genic allele Geno-types analyzed Initial reference MGI ID
Tg(Ipf1-cre)89.1Dam Pdx1-cre 5.5-kb portion of the mouse pdx1 promoter fused to cre cassette Random insertion (pro-nuclear injection Expression of Cre is driven by regulatory elements which regulate pdx1 expression 0 Cre Cre/0 (hemi-zygote); 0/0 (wild-type control) Develop-ment 2002, 129(10):2447-2457 2684317
Tg(CAG-Bgeo/GFP)
21Lbe
Z/EG Ubiquitous recombinant promoter, followed by LoxP-flanked LacZ cassette, followed by GFP cassette Random insertion with screening for high expression in ES cells (ES cell electro-poration) When cre is expressed in a cell, LacZ cassette is excised, leading to GFP expression in that cell and all daughter cells 0 GFP GFP/0 (hemi-zygote) Genesis 2000, 28(3-4):147-155 3046177
Gt(ROSA)26
Sortm1Sor
Rosa26R LoxP-flanked stop cassette followed by LacZ cassette Targeted to the ubiquitously expressing Gt(ROSA)26Sor locus When cre is expressed in a cell, stop cassette is excised, leading to LacZ expression in that cell and all daughter cells + LacZ LacZ/+ (hetero-zygote) Nature Genetics 1999, 21(1):70-71 1861932