Table 1 Transgenic mouse lines and relevant genotypes employed
Formal designation | Common designation | Transgene design | Trans-gene insertion | Purpose | WT allele | Trans-genic allele | Geno-types analyzed | Initial reference | MGI ID |
|---|---|---|---|---|---|---|---|---|---|
Tg(Ipf1-cre)89.1Dam | Pdx1-cre | 5.5-kb portion of the mouse pdx1 promoter fused to cre cassette | Random insertion (pro-nuclear injection | Expression of Cre is driven by regulatory elements which regulate pdx1 expression | 0 | Cre | Cre/0 (hemi-zygote); 0/0 (wild-type control) | Develop-ment 2002, 129(10):2447-2457 | 2684317 |
Tg(CAG-Bgeo/GFP) 21Lbe | Z/EG | Ubiquitous recombinant promoter, followed by LoxP-flanked LacZ cassette, followed by GFP cassette | Random insertion with screening for high expression in ES cells (ES cell electro-poration) | When cre is expressed in a cell, LacZ cassette is excised, leading to GFP expression in that cell and all daughter cells | 0 | GFP | GFP/0 (hemi-zygote) | Genesis 2000, 28(3-4):147-155 | 3046177 |
Gt(ROSA)26 Sortm1Sor | Rosa26R | LoxP-flanked stop cassette followed by LacZ cassette | Targeted to the ubiquitously expressing Gt(ROSA)26Sor locus | When cre is expressed in a cell, stop cassette is excised, leading to LacZ expression in that cell and all daughter cells | + | LacZ | LacZ/+ (hetero-zygote) | Nature Genetics 1999, 21(1):70-71 | 1861932 |