Figure 1

YgfZ is critical for resolving plumbagin toxicity. (A) Growth inhibition assay on the agar diffusion plates. Bacteria harboring the indicated plasmids were plated overnight at 37°C on MH plates in the presence of plumbagin-containing filter discs (8 mm in diameter). (B) Diameters of the inhibition zones seen in (A) at different plumbagin concentrations. Note: strain BW25113 (WT) is the parental strain of the ΔygfZ mutant whereas pMH-ygfZ differs from the promoterless pMH vector by carrying ygfZ as well its upstream promoter region. NS: no significance; * p < 0.05