Figure 2
From: A role of ygfZ in the Escherichia coli response to plumbagin challenge
Different roles played by YgfZ and SodA in counteracting plumbagin. The ΔygfZ and ΔsodA strains were transformed with pQE-sodA and pQE-ygfZ to express SodA and YgfZ, respectively, and the agar diffusion assay was performed similar to that described in legend to Fig. 1. Note: pQE60 was the vector used for expression construction. Inset: the plasmid-encoded Hisx6-tagged proteins were well expressed in the transformants as revealed by Western blotting; antibody-detected DnaK served as a protein-loading control. NS: no significance