Skip to main content
Figure 1 | Journal of Biomedical Science

Figure 1

From: Identification of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a binding protein for a 68-kDa Bacillus thuringiensis parasporal protein cytotoxic against leukaemic cells

Figure 1

A. HPLC Chromatogram of trypsin activated Bt18 parasporal protein. Trypsin activated parasporal protein was applied to a Mono Q exchange column with 20 mM Piperazine buffer (pH 9.8). The column was set to run at 1.0 mL/min with the salt gradient (0-1 M NaCl). B. SDS-PAGE profile of Mono Q purified trypsin activated Bt18 parasporal protein. Coomassie Blue stained SDS 10% polyacrylamide gel. Lane 1: Molecular weight marker; Lane 2: Solubilised and activated Bt18 parasporal protein; Lane 3: HPLC Fraction 3; Lane 4: HPLC Fraction 4; Lane 5: HPLC Fraction 5; Lane 6: HPLC Fraction 6.

Back to article page

Journal of Biomedical Science

ISSN: 1423-0127