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Figure 4 | Journal of Biomedical Science

Figure 4

From: Identification of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a binding protein for a 68-kDa Bacillus thuringiensis parasporal protein cytotoxic against leukaemic cells

Figure 4

Toxin overlay blot with Bt18 parasporal protein. Toxin overlay blot was prepared as described in the methods section. (A): SDS-PAGE gel; (B): western blot. Lane 1: Molecular weight marker; Lane 2: CEM-SS cells lysate; Lane 3: Diluted membrane proteins (hydrophobic fraction); Lane 4: Undiluted membrane proteins (hydrophobic fraction); Lane 5: Hydrophilic fraction; Lane 6: Bt18 parasporal protein; 1 mg/mL; Lane 7: Bt18 parasporal protein; 0.5 mg/mL (Arrow pointing at the binding protein).

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Journal of Biomedical Science

ISSN: 1423-0127