Figure 1
Characterization of the number of E7-specific CD8+ T cells and PADRE-specific CD4+ T cells in vaccinated mice. C57BL/6 mice (5 per group) were immunized subcutaneously using 20 μg/mouse of HPV-16 E7 (aa 49-57) peptide, 20 μg/mouse of PADRE peptide or a combination of the two with or without treatment with 20 μg/mouse of poly(I:C). Mice received a booster dose one week later. One week after the last vaccination, splenocytes from vaccinated mice were harvested and stimulated with the E7 or PADRE peptide. Cells were characterized for E7-specific CD8+ T cells or PADRE-specific CD4+ T cells using intracellular IFN-γ staining followed by flow cytometry analysis. Splenocytes without peptide stimulation were used as negative control. (A) Representative data of intracellular cytokine staining followed by flow cytometry analysis showing the number of E7-specific IFNγ+ CD8+ T cells in the various groups (right upper quadrant). (B) Bar graph depicting the numbers of E7-specific IFN-γ-secreting CD8+ T cells per 3 × 105 pooled splenocytes (mean ± s.d.). (C) Representative data of intracellular cytokine staining followed by flow cytometry analysis showing the number of PADRE-specific IFNγ+ CD4+ T cells in the various groups (right upper quadrant). (D) Bar graph depicting the numbers of PADRE-specific IFN-γ-secreting CD4+ T cells per 3 × 105 pooled splenocytes (mean ± s.d.). Data shown are representative of two experiments performed. * indicates p < 0.05.