Figure 2
Comparison of the number of E7-specific CD8+ T cells and PADRE-specific CD4+ T cells in mice vaccinated with the E7 and PADRE mixture with poly(I:C) versus the E7-PADRE fusion peptide with poly(I:C). C57BL/6 mice (5 per group) were immunized subcutaneously using the mixture of E7 and PADRE peptide with poly(I:C) or the E7+PADRE fusion peptide with poly(I:C). Mice received a booster dose one week later. One week (A & B) or 5 weeks ( C & D ) after the last vaccination, splenocytes from vaccinated mice were harvested and stimulated with the E7 or PADRE peptide. Cells were characterized for E7-specific CD8+ T cells or PADRE-specific CD4+ T cells using intracellular IFN-γ staining followed by flow cytometry analysis. Splenocytes without peptide stimulation were used as negative control. (A) Bar graph depicting the numbers of E7-specific IFN-γ-secreting CD8+ T cells per 3 × 105 pooled splenocytes (mean ± s.d.). (B) Bar graph depicting the numbers of PADRE-specific IFN-γ-secreting CD4+ T cells per 3 × 105 pooled splenocytes (mean ± s.d.). (C) Bar graph depicting the numbers of memory E7-specific CD8+ T cells per 3 × 105 pooled splenocytes (mean ± s.d.). (D) Bar graph depicting the numbers of memory PADRE-specific CD4+ T cells per 3 × 105 pooled splenocytes (mean ± s.d.). Data shown are representative of two experiments performed.