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Table 1 Half-wave potentials of HLMWb5 and its site-specific mutants in comparison with various animal cytochrome b5 and their site-specific mutants.

From: Direct electrochemical analyses of human cytochromes b5 with a mutated heme pocket showed a good correlation between their midpoint and half wave potentials

Samples half-wave potential (mV) (vs. SHE) Electrode references
HLMWb5 -19.5 Au* present study
LMWb5 -21 Au* [32]
full-length human cyt. b5 -20.5 Au* present study
L51I -30.5 Au* present study
A59V -29 Au* present study
A59S -31.5 Au* present study
G67A -40.5 Au* present study
G67S -32 Au* present study
human erythrocyte cyt. b5 -9 Au** [42]
rat OM cyt. b5 (soluble domain) +8 Au* [25]
rat OM cyt. b5 (soluble domain) -40 Au*+Mg2+ [25]
rat OM cyt. b5 (soluble domain) -78 Au*+Cr3+ [25]
rat OM cyt. b5 (soluble domain) -27 Carbon [28]
DiMe OM cyt. b5 (soluble domain) +20 Carbon [28]
V61L/V45L -14 Carbon [28]
rat OM cyt. b5 (soluble domain) -26 ITO [29]
DiMe OM cyt. b5 (soluble domain) +4 ITO [29]
V61I/V45I -24 ITO [29]
rat liver cyt. b5 (soluble domain) +16.2 Au*2 [38]
A67V (soluble domain) -2.8 Au*2 [38]
rat liver cyt. b5 (soluble domain) -7 Au*3 [47]
bovine liver cyt. b5 (tryptic fragment)    
   + 20 mM Mg2+ -6 Au*4 [44]
bovine liver cyt. b5 (tryptic fragment)    
   + 20 mM Cr(NH3)63+ -14 Au*4 [44]
bovine liver cyt. b5 (tryptic fragment) -10 Au*3 [18]
V61E (bovine liver, tryptic) -25 Au*3 [18]
V61Y (bovine liver, tryptic) -33 Au*3 [18]
V61H (bovine liver, tryptic) +11 Au*3 [18]
V61K (bovine liver, tryptic) +17 Au*3 [18]
V45Y -35 Au*3 [48]
V45H +8 Au*3 [48]
V45E -26 Au*3 [48]
  1. The half-wave potentials (E1/2) were measured from respective cyclic voltammogram using various electrodes pre-treated as indicated.
  2. Au*, gold-electrode modified with β-mercaptopropionic acid + poly-L-lysine (200 μM) carbon, DDAB-modified glassy carbon electrode
  3. ITO, indium-doped tin oxide electrode + poly-L-lysine (200 μM)
  4. Au**, gold-electrode modified with KCTCCA peptide
  5. Au*2, gold-electrode modified with HO(CH2)4SH
  6. Au*3, gold-electrode modified with cysteine
  7. Au*4, gold-electrode modified with HSCH2COOH