Table 1 Half-wave potentials of HLMWb₅ and its site-specific mutants in comparison with various animal cytochrome b₅ and their site-specific mutants.

HLMWb₅

-19.5

Au*

present study

LMWb₅

-21

Au*

[32]

full-length human cyt. b₅

-20.5

Au*

present study

L51I

-30.5

Au*

present study

A59V

-29

Au*

present study

A59S

-31.5

Au*

present study

G67A

-40.5

Au*

present study

G67S

-32

Au*

present study

human erythrocyte cyt. b₅

-9

Au**

[42]

rat OM cyt. b₅ (soluble domain)

+8

Au*

[25]

rat OM cyt. b₅ (soluble domain)

-40

Au*+Mg²⁺

[25]

rat OM cyt. b₅ (soluble domain)

-78

Au*+Cr³⁺

[25]

rat OM cyt. b₅ (soluble domain)

-27

Carbon

[28]

DiMe OM cyt. b₅ (soluble domain)

+20

Carbon

[28]

V61L/V45L

-14

Carbon

[28]

rat OM cyt. b₅ (soluble domain)

-26

ITO

[29]

DiMe OM cyt. b₅ (soluble domain)

+4

ITO

[29]

V61I/V45I

-24

ITO

[29]

rat liver cyt. b₅ (soluble domain)

+16.2

Au*²

[38]

A67V (soluble domain)

-2.8

Au*²

[38]

rat liver cyt. b₅ (soluble domain)

-7

Au*³

[47]

bovine liver cyt. b₅ (tryptic fragment)

   + 20 mM Mg²⁺

-6

Au*⁴

[44]

bovine liver cyt. b₅ (tryptic fragment)

   + 20 mM Cr(NH₃)₆³⁺

-14

Au*⁴

[44]

bovine liver cyt. b₅ (tryptic fragment)

-10

Au*³

[18]

V61E (bovine liver, tryptic)

-25

Au*³

[18]

V61Y (bovine liver, tryptic)

-33

Au*³

[18]

V61H (bovine liver, tryptic)

+11

Au*³

[18]

V61K (bovine liver, tryptic)

+17

Au*³

[18]

V45Y

-35

Au*³

[48]

V45H

+8

Au*³

[48]

V45E

-26

Au*³

[48]