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Table 2 Midpoint potentials of human and bovine cytochrome b5 and its site-specific mutants.

From: Direct electrochemical analyses of human cytochromes b5 with a mutated heme pocket showed a good correlation between their midpoint and half wave potentials

Samples Midpoint potentials (mV) (vs. SHE) method References
HLMWb5 -3.2 optical titration present study
HLMWb5 + poly-L-lysine (200 μM) +16.5 optical titration present study
human erythrocyte cytochrome b5 -2 optical titration [46]
full-length human cytochrome b5 -2.6 optical titration present study
full-length human cytochrome b5    
+ poly-L-lysine (200 μM) +8.7 optical titration present study
L51I -9.5 optical titration present study
L51I + poly-L-lysine (200 μM) +10.5 optical titration present study
A59V -7.7 optical titration present study
A59V + poly-L-lysine (200 μM) +11.7 optical titration present study
A59S -4.9 optical titration present study
A59 S + poly-L-lysine (200 μM) +9.6 optical titration present study
G67A -8.4 optical titration present study
G67A + poly-L-lysine (200 μM) -2.7 optical titration present study
G67S -7.3 optical titration present study
G67S + poly-L-lysine (200 μM) +14.2 optical titration present study
bovine liver cyt. b5 (tryptic fragment) +5.1 OTTLE [49]
bovine liver cyt. b5 (tryptic fragment) -1.8 OTTLE [50]
bovine liver cyt. b5 (tryptic fragment) +5 OTTLE [44]
bovine liver cyt. b5 (tryptic fragment)    
+ 20 mM Mg2+ +15 OTTLE [44]
bovine liver cyt. b5 (tryptic fragment) +2 OTTLE [24]
F35L -26 OTTLE [24]
F35H -49 OTTLE [24]
F35Y -64 OTTLE [24]
rat OM cyt. b5 (soluble domain) -102 OTTLE [25]
rat OM cyt. b5 (soluble domain)    
+ poly-L-lysine (104 μM) -70 OTTLE [28]
rat OM DiMe cyt. b5 (soluble domain) -36 OTTLE [28]
rat OM DiMe cyt. b5 (soluble domain)    
+ poly-L-lysine (104 μM) -33 OTTLE [28]
V61L -117 OTTLE [28]
V61L/V45L -148 OTTLE [28]
V61I/V45I -63 OTTLE [29]
  1. Midpoint potentials of human cytochrome b5 (HLMWb5) and its mutants were estimated for the redox titration data obtained in the absence or presence of poly-L-lysine by a least-square curve fitting using the Nernst equation with assuming a single redox component. For a comparative purpose, midpoint potentials for the tryptic fragment of bovine liver cytochrome b5 and OM cytochrome b5 obtained by OTTLE method were presented.
  2. OTTLE, optically-transparent-thin-layer-electrode in the presence of Ru(NH3)6 as a mediator