PiT2 immunostaining in normal mice. A: PiT2 immunostaining in the cortex of a normal mouse. In this representative image, the staining is detected in all cortical layers, with a "rosette like" aspect. The arrow indicates a characteristic stained neuron displayed in the enlarged inset (magnification x300). B: PiT2 immunostaining in the striatum of a normal mouse. Some PiT2-stained cells carry a "rosette like" pattern similar to that observed in the cortex (arrow and enlarged inset, magnification x300). Noteworthy, the white matter tracts are not stained (shown within dotted circles). C: PiT2 immunostaining in the substantia nigra (SN) of a normal mouse. PiT2 staining pattern in SN is comparable to the patterns observed in the cortex and the striatum with a "rosette like" aspect. The cerebral peduncle (white matter) does not show any PiT2 staining. The arrow points at a characteristic stained nigral cell as shown in the inset (magnification x300). D: PiT2 immunostaining in the cerebellum of a normal mouse. Purkinje cells are labelled with the PiT2 specific probe (arrow). Alexa 488 signals for PiT2 (green) and Hoechst signals for the nuclear counterstaining (blue) are merged. CP: cerebral peduncle, SNpr: substantia nigra pars reticulata, ML: molecular layer, GL: granular layer. Scale bar: 100 μm.