Figure 3

pH dependence of the activity of acyl-CoA dehydrogenases in taurine buffer. Conditions: [taurine] = 47 mM, [C₈-CoA] = 100 µM, [C₁₂-CoA] = 35 µM, [C₄-CoA] = 100 µM at 37°C (substrate concentrations are ≥5•Km). Enzyme concentration in the assays was 60 nM. Enzyme activities were estimated using the ferricenium assay [33] and are normalised for the sake of comparison. The activities values (turnover) extrapolated to low and high pH are 560 and 3800 min^-1 for MCAD; 85 and 2400 min^-1 for LCAD and 160 and 2050 min^-1 for SCAD respectively. The pK values are derived from the curve fits. The data points are the average of 3-4 individual measurements and the vertical bars indicate the scatter where appropriate.