Figure 3
Effects of taurine chloramine on transnuclear migration of NF-κB. FLSs were seeded (5 × 106 cells) into 100-mm dishes and grown to 80% confluence. The cells were serum-starved overnight and stimulated by adiponectin (10 µg/ml) or IL-1β (10 ng/ml) for 120 min and 90 min, respectively, in the presence or absence of TauCl (600 µM). The nuclear levels of NF-κB were measured by ELISA detection of p65 in nuclear extracts. TauCl differentially inhibited the level of NF-κB in the nuclei of adiponectin- or IL-1β-stimulated RA FLSs. Values are expressed ± S.E.M. *p<0.05 versus adiponectin- or IL-1β-treated group in the presence of TauCl. ns, not significant.