Figure 4

Immunohistochemistry study of kidney tissues from wild-type and TauT transgenic mice with or without cisplatin treatment. A. Immunostaining for p53, TauT protein, taurine, PUMA, and FasL (B) was performed on kidney tissue slides using specific antibodies against the indicated proteins as described in the Methods. C. TUNEL assay was performed on kidney tissue slides using a TACS TdT DAB in situ apoptosis detection kit (R&D system). Results represent three separate experiments (n=6). Magnification: A x 200, B (top) x 40 and (bottom) x 400, and C x 400.