Effects of a bilateral renal ischemia reperfusion insult on urinary protein excretion. Line graphs show daily total protein excretion in experimental groups as described under Figure 1; data are means ± SEM (n=5-7 animals/group). Inset shows appearance of albumin in urine of the 3 IR groups. Lane 1 shows molecular weight standards (6.5-200kDa) while lane 2 is a sample of bovine serum albumin from comparison (arrow). Lanes 3-10 show urinary protein migration for the experimental groups as follows: 3-4 (sham control), 5-6 (C/IR), 7-8 (TD/IR) and 9-10 (TT/IR). * p<0.05 C/IR and TT/IR compared to the other two groups at the same time point.