Figure 2

Expression of stress proteins and antioxidant enzymes in the lesion center. (A) Western blot analysis shows time course change in the expression levels of MnSOD, Prx1, Prx6, Hsp27, catalase (CAT), Hsp60, DPYL2 and DPYL5. The proteins extracted from the LC of the injured spinal cords at the different survival time points (day 1 and 14) after SCI or sham control. The same blot was stripped and reprobed with anti-ERK44/42 antibody as internal loading control. Relative intensity of the indicated protein level bands normalized to ERK 44 was measured. Data are presented as means ± SEM from three separate experiments. *p < 0.05 versus sham control. (B). The spinal cord was removed at day 8 post SCI from the rats without or receiving control Ad, rAd-SOD, rAd-calatase (CAT), rAd-GPx gene therapy. Horizontal spin cord tissue sections were subjected to in situ apoptosis analysis. Data represent the mean ± SEM. *p < 0.05 versus the control group without Ad injection. ^#p < 0.05 versus the group treated with control Ad.