Improvement of hindlimb locomotor recovery in SCI rats by treatment with chABC. (A). Rats received administration with vehicle (n = 4), or with chABC (0.03 U/injection, 0.06 U/rat) immediately after SCI (n = 4) or at 3 day post SCI (n = 4). To examine hindlimb locomotor function in SCI rats, the BBB open-field analysis was conducted. Data are presented as mean ± SEM. *p <0.05 versus the vehicle-treated group. (B). The spinal cord tissues were collected from SCI rats at day 31. The longitudinal tissue sections containing the LC were subjected to immunofluorescence for GAP-43. Arrowheads indicate the fine fragmented GAP-43-positive neuronal fibers in the vehicle-treated control. Arrows and open arrows show the GAP-43-positive neuronal bundles in chABC-treated group. Scale bar, 100 μm (left panel) and 50 μm (middle and right panels).