The promoter region of the mir-21 gene. 7A. The DNA sequence of mir-21 promoter region was conserved among mouse, rat and human, and a consensus CArG motif was found in the promoter of all three species. 7B. The mouse mir-21 gene sequence corresponding to pri-mir-21 and its promoter region. A CArG motif is 197 bp upstream of mir-21 primary transcript. A pGL3-luciferase construct containing promoter fragment (-586 bp to -3 bp) was generated and used in the present experiment (see details in Materials and Methods).