Figure 6

The potentiation of PI3K-Akt phosphorylation and Bcl-xL expression by the overexpression of FMRP. Cells transfected with wither eGFP- or eGFP-FMRP were treated with ETO (20 μM, 3 hr) and analyzed using Western blot. ETO-induced PI3K (A) and Akt (B) phosphorylation as well as Bcl-xL (C) expression were potentiated as compared with control. * significantly different as compared with control, ^# significantly different as compared with eGFP transfected cells. (D) A specific Akt inhibitor VIII reduced activation of Akt as well as induction of Bcl-xL in a concentration dependent manner. Graphs represent the densitometric quantification of the band intensity. * significantly different as compared with ETO treated cells, ^# significantly different as compared with eGFP transfected cells (p < 0.01, n = 4). eGFP denotes HeLa cells transfected with eGFP-empty vector, eGFP-FMRP indicates cells transfected with eGFP-FMRP vector.