Figure 7
Akt activation is necessary for the pro-survival effects of FMRP overexpression in ETO treated HeLa cells. Cells transfected with wither eGFP- or eGFP-FMRP were incubated with Akt inhibitor VIII (10 μM, 1 hr) before ETO (20 μM, 3 hr) treatment and analyzed by PI staining. (A) Red fluorescence represents PI positive cells and green fluorescence represents GFP positive cells. White arrows indicate yellow spots which represent double positive for PI and GFP. (B) Graphs represent the quantification of the PI/GFP double-positive cell number. * significantly different as compared with eGFP transfected cells, # significantly different as compared with ETO treated HeLa cells (p < 0.01, n = 4). eGFP denotes HeLa cells transfected with eGFP-empty vector, eGFP-FMRP indicates cells transfected with eGFP-FMRP vector.