Figure 1
The effects of dopamine D 2 receptor agonist and antagonist on apoptosis of neonatal cardiomyocytes exposed to simulated ischemia/reperfusion. Cardiomyocytes were subjected to 2 h of simulated ischemia, and then incubated under normal conditions in the presence or absence of 10 μM bromocriptine or haloperidol for 24 h. The data are means ± S.E.M. of five determinations.▼▼P < 0.01 versus control; ★★P < 0.01 versus ischemia/reperfusion. (A) MTT assay for cell viability. (B) DNA fragmentation determined by quantitative TUNEL staining of apoptotic cardiomyocytes. (C) Flow cytometry analysis.