Figure 1
From: Protein kinases modulate store-operated channels in pulmonary artery smooth muscle cells
Cyclopiazonic acid (CPA) evokes an SOC conductance. Using whole cell patch clamp electrophysiology, PASMCs were voltage clamped and then periodically exposed to voltage ramps (-100 mV to +100 mV) in the absence and presence of 10 μM CPA ± 10 μM SKF-96365 (SKF). A, original traces showing that CPA activates whole cell SOC currents in PASMCs. SKF-96365 was added to the perfusate 12 min after CPA. B, individual I-V relationships under resting conditions (a, Control), and in the presence of CPA (b) and SKF-96365 (c). C, mean I-V relationships of whole cell SOC currents. Data are means ± SE, n = 6. * denotes significant difference CPA alone.