Effects of PKC activation and Ins(1,4,5)P
on single channel SOC activity. Using inside out patch clamp electrophysiology, single channel SOC activity in PASMCs was assessed (holding potential, -80 mV) in the absence and presence of 10 μM PMA ± 10 μM Ins(1,4,5)P3. Representative traces and amplitude histograms of single channel currents (a, control; b, PMA; c, PMA + Ins(1,4,5)P
can be found in A &B, respectively. Mean NPo data was plotted in C. Data are means ± SE, n = 6. * and # denote significant difference from control and PMA, respectively.