Figure 5
Characterization of the antigen-specific CD8+ T cell immune responses in mice treated with various vaccines in combination with DMXAA administered at different time points. 5-8 weeks old C57BL/6 mice were vaccinated with CRT/E6 DNA via gene gun, Sig/E7/L1 vaccinia virus intraperitoneally or PADRE DNA vaccine via gene gun delivery and treated with DMXAA at 3 days before vaccination (-3), simultaneously (0) or 3 days after vaccination (+3) as indicated in Figure 3A. One week after last vaccination, splenocytes from mice were harvested and characterized for (A) HPV-16 E6aa50-57-specific CD8+ T cell responses, (B) HPV-16 E7aa49-57-specific CD8+ T cell responses, or (C) PADRE-specific CD4+ T cell responses using intracellular IFN-γ staining followed by flow cytometry analysis. A & B. Bar graph depicting the number of antigen-specific IFNγ+ CD8+ T cells per 3 × 105 splenocytes ± SEM following vaccination +/- DMXAA treatment. C. Bar graph depicting the number of antigen-specific IFNγ+ CD4+ T cells per 3 × 105 splenocytes ± SEM following vaccination +/- DMXAA treatment. The data shown here are from one representative experiment of two performed.